Journal: Pain
Article Title: Spatially resolved single-cell analysis of transcriptomic changes linked with neuropathic pain in human neuromas
doi: 10.1097/j.pain.0000000000003907
Figure Lengend Snippet: HLA-A immunohistochemistry in human neuromas. (A–C) Representative sections of HLA-A (Cy3) dual-labeling with the endothelial cell marker UEA-I (FITC, A), the axonal marker TUJ1 (FITC, B), and the immune cell marker CD45 (FITC, C) in samples of human neuromas. Scale bar: 100 μm. (D and E) Representative sections of HLA-A (Cy3) signal within nerve fascicles, marked by TUJ1 labeling (FITC) in a painful (LN4, D) and nonpainful (LN10, E) sample of human neuroma. Scale bar: 100 μm. (F) Correlation between the area of HLA-A positive staining associated with nerve fascicles (identified with TUJ1 labeling) and the intensity of pain reported by the patient. Pearson r = 0.69, P = 0.0391. FITC, fluorescein isothiocyanate; HLA, human leukocyte antigen; UEA, Ulex europeus agglutinin.
Article Snippet: HLA-A strongly colocalized with UEA-I (Vector Laboratories, FL-1061), a leptin that binds to glycoproteins expressed on the surface of endothelial cells (Fig. A).
Techniques: Immunohistochemistry, Labeling, Marker, Staining